B2R / SIMULATED WESTERN BLOT HANDOFF

All numbers are synthetic. Bands are illustrative and are not used to derive measurements.
ROI area: 100 pixels. Mean background: 30 arbitrary units per pixel.
Corrected target = target raw - 3,000.
Corrected control = control raw - 3,000.
Ratio = corrected target / corrected control.
Relative value = ratio / mean of the three Group A ratios.

Six simulated samples, two groups. Biological/technical replicate identity unspecified.
No error bars, p-values or inferential statistical claims.
An actual project requires original images, lane mapping, exposure/linearity review,
ROI and background review, valid loading-control selection, and a confirmed experimental design.
No assessment here establishes that real experimental images can be quantified reliably.